IMPACT OF FUNGAL CELL SURFACE VARIABILITY UPON FUNGAL INTERACTIONS WITH THE HOST AND MICROBIOTA

Objectives:

    - Define the molecular basis for cell surface variability between C. albicans clinical isolates using genome-wide screens (SNP calling plus RNA sequencing) and targeted molecular dissection of loci highlighted by these screens
    - To determine the influence of host and microbiota signals, and physical contact with microbiota, on fungal surface variability and how this impacts on immune surveillance, colonization and infection outcome. A combination of cell wall biochemistry, flow cytometry, microscopy, ex vivo and in vivo models will be utilised.

Expected Results:

    - Identification of host and microbiota signals and biochemical and biophysical mechanisms that drive surface variability in Candida.
    - Definition of how surface variability affects immune surveillance, colonisation and infection outcome.

PROJECT SUPERVISOR:

CAROL MUNRO