AN EXAGGERATED MONOCYTE-DERIVED CYTOKINE RESPONSE TO CANDIDA HYPHAE

Updated: Nov 16

Published in The Journal of Infectious Diseases – 15/05/2022 Complete title : AN EXAGGERATED MONOCYTE-DERIVED CYTOKINE RESPONSE TO CANDIDA HYPHAE IN PATIENTS WITH RECURRENT VULVOVAGINAL CANDIDIASIS


Diletta Rosati, Mariolina Bruno, Martin Jaeger, Bart-Jan Kullberg, Frank van de Veerdonk, Mihai G Netea, Jaap ten Oever


Background Recurrent vulvovaginal candidiasis (RVVC) affects up to 8% of women. The immunopathogenesis is poorly understood but it has been suggested that RVVC might be due to dysregulated innate immune response. The aim of this study was to compare cytokine profiles in stimulated primary mononuclear cells (PBMCs) from RVVC and healthy individuals.


Methods

PBMCs isolated from RVVC patients (n = 24) and healthy volunteers (n = 30) were stimulated with unspecific and pathogen-specific antigens. Cytokine production was assessed after 24 hours, 48 hours, and 7 days using ELISA.


Results

No significant differences in cytokine production were found in T helper 1 (Th1), Th2, and Th17 immunity in response to both unspecific and pathogen-specific stimulations. Tumor necrosis factor-α (TNF-α) production in response to C. albicans hyphae was significantly higher in patients than controls and within the patient group, a significant positive correlation was found between interleukin-1β (IL-1β) and both TNF-α and IL-6. Both IL-1β/IL-1Ra and TNF-α/IL-10 ratios in Candida hyphae-stimulated PBMCs were significantly higher in patients than controls.


Conclusions

Women affected by RVVC showed increased monocytes-derived cytokine production, which might contribute to an exaggerated vaginal immune response to Candida hyphae. RVVC patients show no defective Th-dependent adaptive immune response upon Candida stimulation.


DOI: 10.1093/infdis/jiaa444